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Simultaneous Estimation of Intracellular Free Mg2+ and pH by Use of a New pH-Dependent Dissociation Constant of MgATP

Jpn J Physiol Vol.52, No.3 pp.323-326
Shinsuke Nakayama, Hideki Nomura, Lorraine M. Smith, Joseph F. Clark
Abstract: In the present technical note for 31P-NMR, we used a new pH-dependent dissociation constant of MgATP, and re-estimated changes in the intracellular free Mg2+ concentration and pH from the chemical shifts of β- and γ-ATP during Na+-removal in smooth muscle. We confirmed the role of Na+–Mg2+ exchange.

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Department of Cell Physiology, Nagoya University Graduate School of Medicine, Nagoya, 466-8550 Japan. h44673a@nucc.cc.nagoya-u.ac.jp
Copyright© 2007 by The Physiological Society of Japan